AIM - Super Broth Base w/o Trace elements

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AIM - Super Broth Base w/o Trace elementsFormula g l Tryptone 35 Yeast extract 20 (NH4)2SO4 3. 3 KH2PO4 6. 8 Na2HPO4 7. 1 Glucose 0. 5 Lactose 2. 0 MgSO4 0. 15 Suspend 74. 85 gram powdered medium in 1 litre distilled water Store dry at room temperature.

20 X sterile RNAse free stock solution

The pH (and pKa at ionic strength I≠0) of the buffer solution changes with concentration and temperature

Chemical formula C5H5N5-O

These Amino acids form a basis for many "Drop-Out" mixtures to select for auxotrophic requirements and transformants

2 at 25°C Suspend 21 gram powdered medium in 1 litre distilled water

Appearance White Crystalline Powder Content by HPLC >97

Cells are grown 1-2 days on pre-sporalation medium before transferring them to sporulation medium

A fine white crystalline quality with excellent properties for cell culture

Weight 181

3 g/L TRIS 1 M 121

9 KH2PO4 15 NaCl 2

Due to the low sodium chloride concentration Formedium™Peptone is well suited for cultivation of yeast cells